Catalog Number - 85318-15P

The 85318‑15P Cogent RP C18 ABX™ HPLC Column delivers maximum‑resolution separations and rugged performance in extreme mobile phase conditions, enabling reliable analysis across a wide pH range (2–12).

Engineered with a bulky, specialized end‑capped C18 phase bonded to high‑purity 3 µm silica, this column minimizes secondary interactions and enhances peak shape for acidic, basic, hydrophobic, and neutral analytes, especially those with very high or very low pKa values.

✅ Ideal as a USP L1 alternate for demanding methods involving strong acids or bases

150 mm Length – Maximum Resolution for Analytical Workflows

• Highest resolving power in the 3 µm 4.6 mm ABX column range

• Maximizes peak capacity for complex mixtures

• Ideal for closely related compounds and difficult separations

• Preferred for final method development, validation, and regulatory workflows

✅ Selected when maximum separation performance is required beyond 50 mm and 100 mm columns

4.6 mm ID – Standard Analytical Platform

• Compatible with standard HPLC systems

• Ideal for:

  • method development

  • QC and validation testing

  • routine analytical workflows

Typical Flow Rate Range

• ~0.8–1.5 mL/min

✅ Provides robust and reproducible analytical performance

3 µm Particle – High Efficiency with Practical Pressure

• More efficient than 5 µm columns

• Produces:

  • Sharper peaks

  • Improved resolution

• Lower backpressure than 1.8 µm UHPLC columns

✅ Ideal for:

• High‑performance HPLC systems

• Resolution improvements without UHPLC requirements

Wide pH Stability (pH 2–12) – Extreme Method Capability

• Designed for high and low pH mobile phase conditions

• Suitable for:

  • Strong acids

  • Strong bases

• Maintains performance under aggressive analytical environments

✅ Enables methods not achievable with standard C18 columns

Why Choose RP C18 ABX™ Columns (3 µm)

Extreme pH Ruggedness

• Stable across full pH range (2–12)

• Ideal for challenging analytes and harsh conditions

Improved Peak Shape for Acids & Bases

• Specialized end‑capping reduces secondary interactions

• Enhances reproducibility and analytical accuracy

Versatile Analytical Performance

• Suitable for hydrophobic, neutral, and polar compounds

• Excellent for long‑chain fatty acids and difficult separations

✅ One of the most popular ABX columns for analytical method development and validation

Application & Performance Advantages

• Provides:

  • Consistent retention and resolution

  • Reliable method transferability

• Comparable performance to other high‑pH C18 columns, including:

  • Advanced high‑pH and rugged C18 phases

✅ Ideal for modernizing and optimizing legacy methods

System & Sample Considerations

• Compatible with standard HPLC systems

• UHPLC compatible if available

Recommended sample preparation:

• 👉 Use 0.2 µm syringe filters for proper sample preparation before injection

✅ Helps:

• Prevent column fouling

• Maintain long‑term performance and accuracy

Typical Applications

• HPLC analytical separations

• Acidic and basic compound analysis

• Pharmaceutical method development and validation

• Stability‑indicating methods

• Hydrophobic and neutral compound analysis

• Complex mixture resolution under extreme pH

Important Usage Notes

• Designed for standard analytical HPLC systems (4.6 mm ID)

• Suitable for extreme pH mobile phases (pH 2–12)

• Ideal for maximum‑resolution workflows and final method validation

• Monitor backpressure relative to instrument capabilities